Thermal inactivation of Byssochlamys nivea in pineapple nectar combined with preliminary high pressure treatments

Byssochlamys nivea is a thermal resistant filamentous fungi and potential micotoxin producer. Recent studies have verified the presence of ascospores of such microorganism in samples of pineapple nectars. Although the majority of filamentous fungi have limited heat resistance and are easily destroyed by heat, Byssochlamys nivea ascospores have shown high thermal resistance. The aim of this work was to evaluate the application of linear and Weibull models on thermal inactivation (70, 80 and 90ºC) of Byssochlamys nivea ascospores in pineapple nectar after pretreatment with high pressure (550MPa or 650MPa during 15min). Following the treatments, survival curves were built up for each processing temperature and adjusted for both models. It was observed that survival curves at 90°C after high pressure pretreatment at 550 MPa/15 min did not fit well to linear and Weibull models. For all the other treatments, the Weibull model presented a better fit. At 90ºC without pressure treatment, the Weibull model also showed a better adjustment, having a larger R2 and a smaller RMSE. Regarding the process effectiveness, a 5-log reduction (t5), as recommended for pasteurization, was only achieved for Byssochlamys nivea ascospores presented in pineapple nectar at 90ºC/10.7 min with previous high pressure treatment of 650 MPa for 15 min. Considering the high intensity and energy demanding process with possibly product damage, other preventive and alternative treatments are being investigated

Does a shell matter for defence? Chemical deterrence in two cephalaspidean gastropodes with calcified shells

Opisthobranch molluscs show an evolutionary trend to reduce, internalize and lose the shell. Many of them base their defensive strategies on natural deterrent products and current evolutionary theory suggests that the acquisition of chemical defences preceded shell reduction and loss, which has characterized the evolution of this group. Here we show that basal, shelled opisthobranch molluscs are defended against sympatric predators even if their protective shell is removed. The cephalaspideans Bulla striata and Haminoea orbignyana, both with distinct shell calcification, significantly deterred feeding by sympatric crab and fish predators, both in laboratory and field assays. However, our results argue against a progressive increment of chemical defences associated with shell reduction, because the cephalaspidean with the more fully calcified shell, Bulla striata, was also the more deterrent. These findings suggest that effective chemical defences might have evolved independently from shell loss, at least in basal opisthobranchs such as cephalaspideans

Design e processo expositivo : criação e comunicação de ambientes expositivos

A necessidade de uma comunicação adequada é essencial no que concerne à transmissão e fruição de uma exposição. O Design possui as ferramentas teóricas e práticas necessárias ao cumprimento desse pressuposto. Com esta investigação, pretendemos destacar a necessidade de uma nova abordagem teórica e programática sobre o processo expositivo, ressaltando a importância dos aspectos tridimensionais e as soluções contextualizantes, que contribuam para o entendimento da exposição como veículo de comunicação. Chamamos especial atenção para o potencial de interlocução do designer de interiores como projectista de ambientes expositivos, tendo em vista a maximização da percepção do visitante. Esta interlocução pode realizar-se através do processo expositivo, sob a forma de histórias organizadas e reduzidas a ícones de significado universal, que são aplicados num espaço real. Aponta-se para a necessidade do desenvolvimento de uma teorização acerca do Design Expositivo, como instância de fundamentação para a teoria comunicacional. Com esta investigação, propõe-se ainda a análise de uma estratégia de reconhecimento da mensagem das exposições, através dos espaços e dos objectos.Abstract: The need for proper communication is essential with regard to transmission and enjoyment of a show. Design has theoretical and practical tools necessary to comply with this assumption. With this research, we intend to highlight the need for a new theoretical and programmatic approach about the exhibition process, highlighting the importance of three-dimensional aspects and contextualizing solutions that contribute to understanding the exhibition as a communication vehicle. We call special attention for the potential of dialogue of the interior designer as an exhibition designer with a view to maximizing the perception of the visitor. This dialogue can take place through the exhibition process, in form of organized stories and reduced to icons of universal significance, which are applied in real space. They point the need to develop a theory about Exhibition Design, as an instance of reasoning for theory communication. This research aims to analyse the strategy of message acknowledge of exhibitions through the spaces and objects

Mentoring Entrepreneurial Networks : mapping conceptions of participants in technological-based business incubators in Brazil

La agenda de la investigación reciente sobre emprendedores incluye el análisis de las estructuras cognitivas de los empresarios de éxito, revelándose como una herramienta importante a la hora de examinar una trayectoria emprendedora. Mediante técnicas de mapas cognitivos, este estudio explora los conceptos de una trayectoria de éxito y la red en sí misma como un todo, para el desarrollo de esta carrera. Fueron estudiados 53 empresarios en siete viveros tecnológicos de la ciudad de Recife, Pernambuco, Brasil. El objetivo específico de este estudio era situar los significados comunes de los emprendedores del vivero teniendo en cuenta las redes de apoyo informal. Este tipo de redes ofrecen apoyo a la carrera empresarial, y el presente estudio examina tanto las características como el modelo conceptual que subyace bajo éstas. La recolección de datos fue realizada por medio de entrevistas a través de la técnica de evocación libre. Los significados comunes indican la existencia de categorías de pensamiento inherentes que fomentan el contexto de la red en el entorno del vivero, especialmente en las redes-mentor. Los resultados refuerzan la interpretación de un modelo mentor informal que emerge de las evocaciones predominantes respecto a una carrera de éxito y de la red en sí misma como promotora de su desarrollo.The recent entrepreneurship research agenda includes the analysis of cognitive structures of successful entrepreneurs, revealing an important tool for the examination of an entrepreneurial career. Using techniques of cognitive maps, this study explores the concepts of a successful career and the network itself, as a whole, for career development. Fifty-three entrepreneurs were studied, in seven technological incubators in the city of Recife, Pernambuco, Brazil. Specifically, this study aimed to map the shared meanings of the incubated entrepreneurs regarding informal support networks. Such networks support the entrepreneurial career and the present study explores the characteristics and the conceptual model that underlies the networks. The data collection was achieved through interviews through a free evocation technique. The shared meanings indicate the existence of inherent thought categories that support network context in the incubator environment, mainly the mentoring networks. The results endorse the interpretation of an informal mentoring model emerging from the dominant evocations concerning a successful career and of the network itself as promoter of career development

Neuroleptic Malignant Syndrome - Diagnostic Challenges in the Emergency Room

Introdução - Síndroma maligna dos neurolépticos (SMN) é um efeito secundário raro potencialmente fatal destes fármacos. Relato de caso - Sexo masculino, 18 anos, encefalopatia não progressiva, atraso global do desenvolvimento, epilepsia, perturbação do comportamento (medicada com haloperidol). Iniciou febre elevada, desidratação, hematemeses e hematúria. Apresentava hipertermia, taquicardia, polipneia, tensão arterial instável, hipertonia generalizada e deterioração da consciência. Laboratorialmente destacava-se neutrofilia, trom bocitopénia, proteína C reactiva ligeiramente aumentada, elevação da creatina-cinase, alterações hepáticas e renais. Sépsis e SMN foram hipóteses de diagnóstico. Apesar da suspensão do haloperidol e início da terapêutica de suporte, ocorreu agravamento progressivo e óbito. Conclusão – Os antipsicóticos são frequentemente utilizados no atraso do desenvolvimento com alterações do comportamento. Os efeitos adversos graves exigem elevado grau de suspeição e início rápido de terapêutica

A unity-based framework for sound transmission and perception in video games

The ability for humans to hear, attend to, and understand incoming sounds is affected, at least, by environmental, morphological, and cognitive factors. Conversely, current implementations of audition in virtual characters often only consider as constraint to the auditory process the distance between the sound emitter and the sound receiver. To cope with this limitation, this paper presents ongoing work on a novel framework integrated in Unity, directed to game developers interested in implementing non-player characters with noisy, character-specific, and context-dependent auditory perception.info:eu-repo/semantics/acceptedVersio

Primary Disorders of Neurotransmitter Metabolism: Experience of a Tertiary Center

Neurotransmitter diseases are a group of inherited disorders attributable to a disturbance of neurotransmitter metabolism. Biogenic amines are neurotransmitters with multiple roles including psychomotor function, hormone secretion, cardiovascular, respiratory and gastrointestinal control, sleep mechanisms, body temperature and pain. Given the multiple functions of monoamines, disorders of their metabolism comprise a wide spectrum of manifestations, with motor dysfunction being the most prominent clinical feature. Methods: Case review of 12 patients from 4 families, with primary disorders of biogenic amine metabolism. Results: Aromatic L-amino acid decarboxylase deficiency (4 patients from 2 families), and GTP-cyclohydrolase (8 patients from 2 families) were the two diseases identified. Age at first symptoms varied between 2 months and 6 years. Developmental delay was present in all cases except 2 patients with GTP cyclohydrolase deficiency. The combination of axial hypotonia and limb dystonia was also frequent. Children with aromatic L-amino acid decarboxylase deficiency exhibited temperature instability, oculogyric crisis and disturbances of sleep. The index case of one family with GTP cyclohydrolase deficiency presented with Parkinsonism (bradykinesia, rigidity and hypomimia). Analysis of neurotransmitters and their metabolites in CSF was crucial for the identification of index cases. Response to therapy was variable but in general unsatisfactory except in a family with GTP cyclohydrolase deficiency. Conclusions: These disorders should be considered in the differential diagnosis of paediatric neurodegenerative diseases, in order to allow an adequate therapeutic trial that can favor prognosis

Zero-bias conductance peak and Josephson effect in graphene-NbTiN junctions

We report electronic transport measurements of graphene contacted by NbTiN electrodes, which at low temperature remain superconducting up to at least 11 Tesla. In devices with a single superconducting contact, we find a more than twofold enhancement of the conductance at zero bias, which we interpret in terms of reflectionless tunneling. In devices with two superconducting contacts, we observe the Josephson effect, bipolar supercurrents and Fraunhofer patterns.Comment: 6 pages, 5 figure

Proteomics for Biomarker Discovery for Diagnosis and Prognosis of Kidney Transplantation Rejection

Renal transplantation is currently the treatment of choice for end-stage kidney disease, enabling a quality of life superior to dialysis. Despite this, all transplanted patients are at risk of allograft rejection processes. The gold-standard diagnosis of graft rejection, based on histological analysis of kidney biopsy, is prone to sampling errors and carries high costs and risks associated with such invasive procedures. Furthermore, the routine clinical monitoring, based on urine volume, proteinuria, and serum creatinine, usually only detects alterations after graft histologic damage and does not differentiate between the diverse etiologies. Therefore, there is an urgent need for new biomarkers enabling to predict, with high sensitivity and specificity, the rejection processes and the underlying mechanisms obtained from minimally invasive procedures to be implemented in routine clinical surveillance. These new biomarkers should also detect the rejection processes as early as possible, ideally before the 78 clinical outputs, while enabling balanced immunotherapy in order to minimize rejections and reducing the high toxicities associated with these drugs. Proteomics of biofluids, collected through non-invasive or minimally invasive analysis, e.g., blood or urine, present inherent characteristics that may provide biomarker candidates. The current manuscript reviews biofluids proteomics toward biomarkers discovery that specifically identify subclinical, acute, and chronic immune rejection processes while allowing for the discrimination between cell-mediated or antibody-mediated processes. In time, these biomarkers will lead to patient risk stratification, monitoring, and personalized and more efficient immunotherapies toward higher graft survival and patient quality of life.info:eu-repo/semantics/publishedVersio